Vascularization and Adipogenesis of a Spindle Hierarchical Adipose-Derived Stem Cell/Collagen/ Alginate-PLGA Construct for Breast Manufacturing
Xiaohong Wang1, Jiayin Wang2
1Prof. Xiaohong Wang, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China.
2Mr. Jiayin Wang, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China.
Manuscript received on 15 January 2015 | Revised Manuscript received on 21 January 2015 | Manuscript Published on 30 January 2015 | PP: 1-8 | Volume-4 Issue-8, January 2015 | Retrieval Number: H1943014815/15©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: The creation of vascularized adipose tissues is a subject of broad fundamental and technological interest in implantable breast manufacturing. Here, we demonstrated a simple, easy, and effective way to fabricate a spindle hierarchical poly (DL-lactic-co-glycolic acid) (PLGA) encapsulated adipose-derived stem cell (ADSC)/collagen/alginate construct with a multiple-branched vascular network using a rotational combined mould system (RCMS). Both the optimized PLGA overcoat and internal collagen/alginate hydrogel provided the ADSCs with a stable and comfortable accommodation to grow, proliferate, and differentiate. Cell viability remained at a comparatively high level during 4 weeks in vitro engagement with two groups of cell growth factor combinations. At the end of the second week, part of the cells were engaged into endothelial cells, while at the end of the fourth week more than 63% of the ADSCs were replaced by adipose cells. We envisage that this RCMS, vascularization and adipogenesis techniques will provide an enabling platform for a wide array of research and clinical applications.
Keywords: Vascularization; adipogenesis; a rotational combined mould system; adipose-derived stem cells (ADSCs); collagen/alginate hydrogel.
Scope of the Article: Manufacturing IoT