3-D Wire Cloth Electrode for Higher Throughput DielectrophoreticSeparation of Bacterial Cell
Fadhila Nadzri1, Zurina Zainal Abidin2, Jamil Mohd Salleh3
1Fadhila Nadzri, Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang, Selangor.
2Zurina Zainal Abidin, Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang, Selangor.
3Jamil Mohd Salleh, Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang, Selangor.
Manuscript received on 08 April 2019 | Revised Manuscript received on 15 April 2019 | Manuscript Published on 26 July 2019 | PP: 381-385 | Volume-8 Issue-6S4 April 2019 | Retrieval Number: F10770486S419/19©BEIESP | DOI: 10.35940/ijitee.F1077.0486S419
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open-access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Dielectrophoresis (DEP) is one of an alternative way for cell separation. It has mainly been limited to processing small volumes due to constrain in fabrication of microelectrode over large surface areas. This work incorporated the wire cloth electrode fabricated using textile technology into a high throughput chamber experiment. The plain-weave wire cloth consists of 71µm stainless steel wires as the microelectrode arrays hold together by polyester yarn warp. This work determines the cell separation yield with parameters on applied voltage, flow rate and cell concentration as well as its optimized variables on the chamber width of 1.2cm and 2.5cm. The optimum voltage achieved was 30Vpk-pk, with flow rate of 3.5 ml/min and maximum cell concentration of 2.08×107 cells/ml. In chamber width comparison, 1.2cm width chamber gives better total percentage yield of 96% than the 2.5cm width chamber of 85% total percentage yield.
Keywords: Dielectrophoresis, Large Scale, Wire Cloth.
Scope of the Article: Bio-Science and Bio-Technology