Biosorption of Heavy Metals from Soil by Pseudomonas Aeruginosa
Vinod Nagashetti1, Mahadevaraju. G.K2, T.S. Muralidhar3, Aamir Javed4, Dwijendra Trivedi5, Khum Prasad Bhusal6
1Vinod Nagashetti, Department of Chemical Engineering, Dayananda Sagar College of Engineering, Bangalore (Karnataka), India.
2Mahadevaraju.G.K, Department of Chemical Engineering, Dayananda Sagar College of Engineering, Bangalore (Karnataka), India.
3T.S.Muralidhar, P.G. Department of Biotechnology, R&D Centre, Dayananda Sagar College of Biological Sciences, Kumaraswamy Layout, Bangalore (Karnataka), India.
4Aamir Javed, P.G. Department of Biotechnology, R&D Centre, Dayananda Sagar College of Biological Sciences, Kumaraswamy Layout, Bangalore (Karnataka), India.
5Dwijendra Trivedi, P.G. Department of Biotechnology, R&D Centre, Dayananda Sagar College of Biological Sciences, Kumaraswamy Layout, Bangalore (Karnataka), India.
6Khum Prasad Bhusal, P.G. Department of Biotechnology, R&D Centre, Dayananda Sagar College of Biological Sciences, Kumaraswamy Layout, Bangalore (Karnataka), India.
Manuscript received on 10 May 2013 | Revised Manuscript received on 18 May 2013 | Manuscript Published on 30 May 2013 | PP: 22-24 | Volume-2 Issue-6, May 2013 | Retrieval Number: F0758052613/13©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Pseudomonas aeruginosa is known to accumulate metal in their cell by the process called Biosorption. This property is used here for removal of metals from the soil to reduce its toxicity. Soil sample collected from Durgapur, West Bengal was mixed with different concentration of metals; chromium, copper & zinc in ppm level and was inoculated with bacterial sample. After incubation the metals were extracted and were compared with different standards using spectrophotometric and titration based methods. Metals are indispensable constituents of approximately one third of all proteins. As such, metals are involved in virtually all biological processes, including metabolism, energy transduction, gene expression, cell signalling, and formation of endo- and exoskeletons, and electron transfer. Among the techniques suitable for the quantification of metal ions in soil sample, inductively coupled plasma mass spectrometry are likely to be the most widely employed. However, although these techniques are reliable and sensitive, they suffer from the limitation of being rather costly (considering instrument acquisition and maintenance), timeconsuming (with respect to sample preparation), and not always readily available. Therefore a general spectrophotometric and titration based analysis is been performed. The results are quite promissable, Metal absorbed by pseudomonas aeruginosa i.e., Cr, Zn and Cu in 1000 ppm were 1.07mg, 1.33mg and 0.67mg when compared to control soil sample in 1000 ppm with metals, but without Pseudomonas aeruginosa , compared were 3.7mg respectively. The present study depicts that bacteria removes chromium efficiently and this could be used for industrial waste management and other environmental contaminants.
Keywords: Biosorption, Pseudomonas Aeruginosa, Chromium, Copper & Zinc In Ppm Level, Industrial Waste Management.
Scope of the Article: Bio-Science and Bio-Technology