Protease Production by Newly Screened Strain Isolated from Carnivorous Plants
Seok-Cheol Cho1, Wan-Young Yoon2
1Seok-Cheol Cho, Division of Food & Pharmaceutical Science and Engineering, Seowon University, Cheongju, Korea.
2Wan-Young Yoon, Department of Clinical Exercise Physiology, Seowon University, Cheongju, Korea.
Manuscript received on 01 January 2019 | Revised Manuscript received on 06 January 2019 | Manuscript Published on 07 April 2019 | PP: 150-153 | Volume-8 Issue- 3C January 2019 | Retrieval Number: C10400183C19/2019©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open-access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Carnivorous plants have various mechanisms for uptake the nutrients of insects. Among them some parasiticbacteria produce protease which has the role of digest the insects. Protease producingbacteriawere isolated from four kinds of carnivorous plant. Selective media including skim milk or sodium caseinate were used for select protease activity. Nine bacteria were identified by 16s rRNA partial sequencing analysis method andfinally choose the eight bacteria which have protease activity. Eight bacteria cultivated at the commercial media for optimizing cell growth and protease activity. All eight isolated strains from carnivorous plants showed higher cell density in R2A broth. In R2A broth, effect of culture temperature for cell growth and protease activity of isolated strains was tested. Temperature range of 20-42℃all strains cultured well and appeared protease activity for 24 hrs incubation. But maximum cell growth and enzyme activity were gained at 37℃ in strain C4. In order to optimize the protease production, cell growth profile and enzyme activity of C4 strain were investigated during incubated at 37℃for 36 hrs. Logarithmic cell growth occurred at 6 hrs and maximum protease activity was gained at 24 hrs but suddenly decreased at 27 hrs. Optimum protease activity gained at C4 strain incubated at 37℃ for 24 hrs and protease activity maintained at the rage of 30-40℃ and decreased suddenly at the below 20℃. Compared with temperature, pH condition was more affected to protease activity, so application of this enzyme must be considered the pH condition. Protease activity did not change at the range of pH 6.0-7.0. Storage temperature of 4℃and 25℃ rarely affected protease activity for 1 month but activity was rapidly decreased at 30℃. Further study on the protease which produced by newly isolated strain in the area of enzyme purification and application to cosmetics for removing dead skin were needed.
Keywords: Carnivorous Plant, Microparasite, Protease, Bacteria Parasitic, rRNA Partial Sequencing.
Scope of the Article: Numerical Modelling of Structures