Association of Alcaligenes Faecalis Strain in Juvenile Earthworms, from Cocoons of Eudrilus Eugeniae
Ganapathy Nadana Raja Vadivu1, S. Sheik Asraf2, Karuppaiah Palanichelvam3
1Ganapathy Nadana Raja Vadivu, Department of Biotechnology, School of Bio and Chemical Engineering, Kalasalingam Academy of Research and Education, Krishnakoil (Tamil Nadu), India.
2S. Sheik Asraf, Department of Biotechnology, School of Bio and Chemical Engineering, Kalasalingam Academy of Research and Education, Krishnakoil (Tamil Nadu), India.
3Karuppaiah Palanichelvam, Department of Biotechnology, School of Bio and Chemical Engineering, Kalasalingam Academy of Research and Education, Krishnakoil (Tamil Nadu), India.
Manuscript received on 06 December 2019 | Revised Manuscript received on 18 December 2019 | Manuscript Published on 30 December 2019 | PP: 688-692 | Volume-9 Issue-2S2 December 2019 | Retrieval Number: B11661292S219/2019©BEIESP | DOI: 10.35940/ijitee.B1166.1292S219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open-access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Cocoons of earthworm Eudrilus eugeniae were collected from vermiculture bed and found that it had antibacterial activity. The size of zone of inhibition was directly proportional to the size of cocoons examined. Along with nutritious fluid and embryos, culturable bacterial community was found inside the cocoons. Bacterial colonies were isolated from the trails of newly hatched, juvenile worms in the nutrient agar medium and examined. Gram negative, rod shaped bacterium was found to be abundant in the trails of juvenile earthworms. Polymerase chain reaction was performed from this bacterium to amplify the gene of 16S rRNA and analyzed. Subsequent bi-directional DNA sequencing revealed that this abundant bacterium is highly related to 16S rRNA gene sequence of a strain, Alcaligenes faecalis. Based on available literature, we hypothesize that this bacterium could be symbiotically associated with cocoons of earthworms.
Keywords: Earthworm, Eudrilus Eugeniae, Cocoon, Alcaligenes Faecalis, 16S rRNA Sequence.
Scope of the Article: Bio-Science and Bio-Technology