Kinetic of 4-Chlorophenols Biodegradation by Arthrobacter Chlorophenolicus A6
Naresh Kumar Sahoo
Naresh Kumar Sahoo, Department of Chemistry, Environmental Science and Technology Programme, ITER, Siksha‘O’Anusandhan Deemed to be University, Bhubaneswar (Odisha), India.
Manuscript received on 06 October 2019 | Revised Manuscript received on 20 October 2019 | Manuscript Published on 26 December 2019 | PP: 252-255 | Volume-8 Issue-12S October 2019 | Retrieval Number: L107010812S19/2019©BEIESP | DOI: 10.35940/ijitee.L1070.10812S19
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open-access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Chlorophenols are listed as priority pollutants both by European community and US EPA. Biodegradation of p-chlorophenol (4-CP) was investigated in batch shake flasks by Arthrobacter chlorophenolicus A6 at initial 4-CP concentrations between 25 to 350 mgl-1. The rate of 4-CP removal decreased with increasing initial 4-CP concentrations due to toxic effects on the microorganisms. The growth and biodegradation kinetic of the culture was evaluated. The batch growth profile of the A chlorophenolicus A6 followed substrate inhibition kinetics with the estimated biokinetic parameters of Ksi = 272 mgl-1, Ks = 65 mgl-1 for 4-CP respectively. High inhibition constant (KSI = 272 mg -l) with a KSI/KS ratio of 4.18 indicates superior 4-CP biodegradation potential of the A chlorophenolicus A6. The maximum rate of 4-CP degradation has been achieved at an optimum substrate concentration of Smax = (KSKSI)1/2 = (65×272)1/2 = 133 mgl−1.
Keywords: Biodegradation, Arthrobacter Chlorophenolicus A6, 4-Chlorophenol, Substrate Inhibition Kinetics.
Scope of the Article: Bio-Science and Bio-Technology