Using Oleaginous Yeast in Spent Tea for Determination of Lipid Accumulation Capacity
E. Sakthivel1, M. Deepak2, N. Mahesh Kumar3, S Jaisurya4
1Mr. E. Sakthivel*, Assistant Professor, Department of Civil Engineering, Karpagam College of Engineering, Coimbatore, India.
2Mr. M. Deepak, Assistant Professor, Department of Civil Engineering, Karpagam College of Engineering, Coimbatore, India.
3Mr. N. Mahesh Kumar, UG student, Department of Civil Engineering, Karpagam College of Engineering, Coimbatore, India.
4Mr. S Jaisurya, UG Student, Department of Civil Engineering, Karpagam College of Engineering, Coimbatore, India.
Manuscript received on April 20, 2020. | Revised Manuscript received on April 30, 2020. | Manuscript published on May 10, 2020. | PP: 360-363 | Volume-9 Issue-7, May 2020. | Retrieval Number: G5268059720/2020©BEIESP | DOI: 10.35940/ijitee.G5268.059720
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Alternative energy becomes the most chief mover of economic growth and plays an important role in this warming world. The whole world comes across the depletion of fossil fuels and has the degradation of the environment. Due to the depletion of fossil fuels, demand is also considerable increasing in demand and rapid increase in the petroleum prices. So, there is a need in search for other alternatives to fossil fuels. Biodiesel is considering being new alternatives to explore our dependency on oil imports that are help in protecting the environment towards the sustainable development. In this study, oleanginous yeast such as Lipomyces starkeyi, Metschnikowia pulcherrima, and Yarrowia lipolytica was cultured where spent tea wastes were used as a substrate. Depolymerization techniques like acid and alkaline treatments were carried out on the spent tea waste substrate. The initial qualitative and quantitative analysis of glucose were carried out by UV spectroscopy gave a maximum result on alkaline treatment. Hence alkaline treatment was selected for culturing and the various oleaginous yeasts were inoculated in the alkali depolymerized samples. After growth of 7 days, biomass was separated from media and was found to be 10.5 g/l, 10 g/l, 8.75 g/l for Y. lipolytica, L. starkeyi and M. pulcherrima. Lipids were isolated from biomass by Bligh and dryer method. Lipid confirmation was sorted out using FTIR.
Keywords: Alternative energy, Biodiesel, Depolymerization techniques, UV Spectroscopy.
Scope of the Article: RF Energy Harvesting