Validation of White Spot Syndrome Virus Infection in penaeid Shrimp of Different Geographical Regions using Electrochemical Immunosensor
Natarajan Anusha1, Annamalai Senthil Kumar2, Raja Sudhakaran3
1Natarajan Anusha, Aquaculture Biotechnology Laboratory, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore (Tamil Nadu), India.
2Annamalai Senthil Kumar, Nano and Bioelectrochemistry Research Laboratory, School of Advanced Sciences, Vellore Institute of Technology, Vellore (Tamil Nadu), India.
3Dr. Raja Sudhakaran, Aquaculture Biotechnology Laboratory, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore (Tamil Nadu), India.
Manuscript received on 01 May 2019 | Revised Manuscript received on 15 May 2019 | Manuscript published on 30 May 2019 | PP: 2475-2481 | Volume-8 Issue-7, May 2019 | Retrieval Number: G5255058719/19©BEIESP
Open Access | Ethics and Policies | Cite | Mendeley | Indexing and Abstracting
© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: WSSV being a major threat causing disease in the aquaculture industry is a well-known pathogen, yet there is no proper treatment found to eradicate the virus causing huge economic loss to the farmers. Being lethal and quickly replicative, the morality occurs within 3-10 days post infection. To support the farmers on field level through detection technique, we had developed an electrochemical immunosensor for WSSV using modified GCE (glassy carbon electrode) immobilised methylene blue. In this study, we had collected shrimp samples from different geographical regions of east coast of India to validate the developed immunosensor method in comparison with other molecular methods and commercially available detection kit. The immunosensor developed is observed to be sensitive and has no controversy with other molecular methods; having the potential to be analysed on site at shrimp culture ponds. Validation of the method is done with different samples and further research is required to make it as a gadget.
Keyword: WSSV, Immunosensor, Diagnosis, Validation.
Scope of the Article: Big Data Quality Validation