Extraction of β 1,3-1,4 Glucanase from Bacillus Subtilus: Aqueous Two-Phase Extraction Versus Salt Precipitation
Pavithra M K S1, Jayapriya A2, Ismathroshan B M3, Kokila S4
1Pavithra M K S, Department of Bio Technology, Bannari Amman Institute of Technology, Sathyamangalam (Tamil Nadu), India.
2Jayapriya A, Department of Bio Technology, Bannari Amman Institute of Technology, Sathyamangalam (Tamil Nadu), India.
3Ismathroshan B M, Department of Bio Technology, Bannari Amman Institute of Technology, Sathyamangalam (Tamil Nadu), India.
4Kokila S, Department of Bio Technology, Bannari Amman Institute of Technology, Sathyamangalam (Tamil Nadu), India.
Manuscript received on 05 January 2019 | Revised Manuscript received on 13 January 2019 | Manuscript published on 30 January 2019 | PP: 107-109 | Volume-8 Issue-3, January 2019 | Retrieval Number: C2587018319/19©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: The activity of extracellular enzyme, β-1,3-1,4 glucanase extracted from Bacillus subtilis was validated on 0.5% starch agar plate The enzyme from the crude broth was extracted using two different methods i) Ammonium Sulphate Precipitation ii) Aqueous two-phase extraction. Ammonium sulphate precipitation was carried out using 65% of the salt. Aqueous two phase extraction was carried out using PEG/ Ammonium Sulphate system using two different concentration of the salt as two different trials. From the study, it was found that extraction of the enzyme using aqueous two-phase process yields high enzyme concentration and increasing the PEG concentration increases the yield of the enzyme.
Keyword: β-1,3-1,4 Glucanase, Ammonium Sulphate Precipitation, Aqueous Two-Phase Extraction, Starch Plate, Halo Zone.
Scope of the Article: Bio-Science and Bio-Technology