Effect of Smoke Exposure on Chronic Inflammation and P53 Expression in Pelvis Epithelial
Kurnia Penta Seputra1, Akmal Fawzi Yusril Umam2, Besut Daryanto3, Taufiq Nur Budaya4, Anita Kenty Watri5
1Kurnia Penta Seputra, Departement of Urology, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
2Akmal Fawzi Yusril Umam, Departement of Urology, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
3Besut Daryanto, Departement of Urology, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
4Taufiq Nur Budaya, Departement of Urology, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
5Anita Kenty Wantri, Departement of Pathology Anatomy, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
Manuscript received on 11 January 2020 | Revised Manuscript received on 07 February 2020 | Manuscript Published on 20 February 2020 | PP: 282-288 | Volume-9 Issue-3S January 2020 | Retrieval Number: C10650193S20/2020©BEIESP | DOI: 10.35940/ijitee.C1065.0193S20
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open-access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Many risk factors can cause upper urinary tract carcinoma. Smoking is the most influential risk factor and is associated with the formation of aromatic acids and could induce apoptotic cycle. This research was aimed to examine the effect of cigarete smoke exposure on inflammatory state and p53 expression in renal pelvis epithelial of wistar rats. As many as 25 male rats aged 6-8 weeks were divided into five groups as follows: Control (without treatment); CS-1, CS-2, CS-4, and CS-8 (treated with CS 1x, 2x, 4x, and 8x/day, respectively). Each exposure was done for 15 minutes for 60 days. histopathological changes were evaluated from HE-stained specimens and immunohistochemistry method was applied to measure p53 expressio neutrophile and lymphocyte count in CS-8 was significantly higher as compared to CS-1 (p<0.05) and control (p<0.05). p53 expression was found in the CS-8 (3 out of 5 subjects had positive p53 expression, with a total of 4-7 cells from 10 hpf). Correlation study showed significant correlation between frequency of cigarette smoking exposure and neutrophile count (p=0.000; r=-0.856); lymphocyte count (p=0.000; r=0.985); and p53 expression (p=0.000; 0.072). We concluded that there were significant differences in acute inflammation state, chronic inflammation state and p53 expression among groups. Correlation study showed that frequency of cigarette smoking exposure was positively correlated with lymphocyte count and p53 expression.
Keywords: Chronic Inflammation, Cigarette Smoke, Renal Pelvis, p53.
Scope of the Article: Bio-Science and Bio-Technology