Influence of Dextransucrase of Weissella Cibaria Nitcsk4 on Low Molecular Weight Dextran Yield: a Statistical Approach using Mixed Level Taguchi Design and Artificial Neural Network
Kanimozhi J.1, Sivasubramanian V.2, Ganesh Moorthy I.3, R. Sivashankar4
1Kanimozhi J., Department of Bio and Chemical Engineering Biotechnology, Kalasalingam Academy of Research and Education Krishnankoil (Tamil Nadu), India.
2Sivasubramanian V., Department of Chemical Engineering, National Institute of Technology Calicut, Kozhikode (Kerala), India.
3Ganesh Moorthy I., Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar (Tamil Nadu), India.
4R. Sivashankar, Department of Chemical Engineering, Hindustan Institute of Science and Technology, Chennai (Tamil Nadu), India.
Manuscript received on 06 December 2019 | Revised Manuscript received on 18 December 2019 | Manuscript Published on 30 December 2019 | PP: 657-664 | Volume-9 Issue-2S2 December 2019 | Retrieval Number: B11611292S219/2019©BEIESP | DOI: 10.35940/ijitee.B1161.1292S219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open-access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: In the present study, the influence of dextransucrase of Weissella cibaria NITCSK4 (DSWc4), sucrose concentration, and reaction temperature on the yield of low molecular weight dextran (LMWD-DexWc4) was investigated using mixed level Taguchi design and back propagation neural network (BPNN). BPNN model with three neurons in a hidden layer generated a low mean squared error (MSE). The determination coefficients (R2 -value) for ANN and Taguchi models were 0.991 and 0.998, respectively. Considering absolute average deviation (AAD) and MSE, Taguchi model is more adequate. Among three factors, the percentage yield of low molecular weight of dextran is invariably dependent on the sucrose concentration. The study suggested that a low sucrose concentration (3% w/v), DSWc4 (0.25 IU/ml) and slightly high temperature (35°C) ultimately favored the production of LMWD-DexWc4 (91.639%). LMW-DexWc4 produced by DSWc4 at optimized conditions was analyzed. The weight average molecular weight of LMW-DexWc4 was calculated using M-H expression, found to be 85775 (≈90 kDa). The relative percentage error between the number and weight average molecular weight was found to be less (4.42%). The polydispersity (PD) index of the LMW-DexWc4 was found to be 0.9576 and the value is close to 1. The PD value depicted that the molecular weight distribution of dextran was narrowly dispersed.
Keywords: Taguchi Orthogonal Array, ANN, Dextran, Dextransucrase, Weissella Cibaria NITCSK4.
Scope of the Article: Ubiquitous Networks