Technology Development to Suppress the Campylobacter Jejuni in Intestinal Microflora of The Broilers by Lactobacilli
Anna Balykina1, Ilya Nikonov2, Yuri Kuznetsov3, Alexander Lunegov4, Alesia Bakhta5
1Anna Balykina*, St. Petersburg State Academy of Veterinary Medicine, Saint-Petersburg, Russia.
2Ilya Nikonov, Perm State Agro-Technological University named after Academician D.N. Pryanishnikov, Perm, Russia.
3Yuri Kuznetsov, St. Petersburg State Academy of Veterinary Medicine, Saint-Petersburg, Russia.
4Alexander Lunegov, St. Petersburg State Academy of Veterinary Medicine, Saint-Petersburg, Russia.
5Alesia Bakhta, St. Petersburg State Academy of Veterinary Medicine, Saint-Petersburg, Russia.
Manuscript received on October 12, 2019. | Revised Manuscript received on 22 October, 2019. | Manuscript published on November 10, 2019. | PP: 1619-1622 | Volume-9 Issue-1, November 2019. | Retrieval Number: A4583119119/2019©BEIESP | DOI: 10.35940/ijitee.A4583.119119
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: This article presents a method for determining the microflora of the blind processes of the intestines of broiler chickens receiving heterologous lactobacilli with anti-C.jejuni activity as a feed additive in comparison with intact chickens. In the course of a scientific and production experiment on the Cobb 500 cross broilers, an experiment was carried out to determine the total number of bacteria in the intestinal contents by quantitative PCR. Sequencing was performed on a MiSeq genomic sequencer (Illumina, Inc., USA) using the MiSeq Reagent Kit v3 kit (Illumina, Inc., USA). Data were obtained on the total number of bacteria and their taxonomic diversity. As a result of the studies, it was shown that the microflora of broilers throughout the entire maintenance period corresponded to the norm. The use of feed additives based on lactobacilli in general had a positive effect on the state of microflora of the blind processes of the intestine.
Keywords: Probiotics, Lactobacilli, Broilers, Campilobacter Jejuni Antagonism, NGS, Real-time Quantitative PCR.
Scope of the Article: Bio-Science and Bio-Technology